High-speed super-resolution microscopy via temporal compression
New high-speed super-resolution imaging technique resolves a longstanding contradiction between spatial resolution and imaging speed
- Date:
- March 10, 2023
- Source:
- SPIE--International Society for Optics and Photonics
- Summary:
- Recently, a research team resolved the contradiction between spatial resolution and imaging speed in optical microscopy. They achieved high-speed super-resolution by developing an effective technique termed temporal compressive super-resolution microscopy (TCSRM). TCSRM merges enhanced temporal compressive microscopy with deep-learning-based super-resolution image reconstruction. Enhanced temporal compressive microscopy improves the imaging speed by reconstructing multiple images from one compressed image, and the deep-learning-based image reconstruction achieves the super-resolution effect without reduction in imaging speed. Their iterative image reconstruction algorithm contains motion estimation, merging estimation, scene correction, and super-resolution processing to extract the super-resolution image sequence from compressed and reference measurements.
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As an indispensable tool for observing the microcosmos, optical microscopy has boosted the development of various fields, including biology, medicine, physics, and materials. However, optical diffraction imposes a spatial resolution restriction on optical microscopy, which hampers exploration of finer structures.
To overcome the resolution limitation, various super-resolution microscopy techniques based on diverse principles have been proposed. Yet these techniques commonly acquire super-resolution at the expense of reduced imaging speed, so achieving high-speed super-resolution imaging that can detect fast dynamics with fine structures has remained a great challenge.
Recently, a research team from East China Normal University, Shenzhen University, and Peking University resolved the contradiction between the spatial resolution and imaging speed. As reported in Advanced Photonics, they achieved high-speed super-resolution by developing an effective technique termed temporal compressive super-resolution microscopy (TCSRM). TCSRM merges enhanced temporal compressive microscopy with deep-learning-based super-resolution image reconstruction. Enhanced temporal compressive microscopy improves the imaging speed by reconstructing multiple images from one compressed image, and the deep-learning-based image reconstruction achieves the super-resolution effect without reduction in imaging speed. Their iterative image reconstruction algorithm contains motion estimation, merging estimation, scene correction, and super-resolution processing to extract the super-resolution image sequence from compressed and reference measurements.
Their studies verified the high-speed super-resolution imaging ability of TCSRM in theory and experiment. To demonstrate the imaging capability of TCSRM, they imaged flowing fluorescent beads in a microchannel, achieving a remarkable frame rate of 1200 frames per second and spatial resolution of 100 nm.
According to corresponding author Shian Zhang, Professor and Deputy Director of the State Key Laboratory of Precision Spectroscopy at East China Normal University, "This work provides a powerful tool for the observation of high-speed dynamics of fine structures, especially in hydromechanics and biomedical fields, such as microflow velocity measurement, organelle interactions, intracellular transports and neural dynamics." Zhang adds, "The framework of TCSRM can also offer guidance for achieving higher imaging speed and spatial resolution in holography, coherent diffraction imaging, and fringe projection profilometry."
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Materials provided by SPIE--International Society for Optics and Photonics. Note: Content may be edited for style and length.
Journal Reference:
- Yilin He, Yunhua Yao, Dalong Qi, Yu He, Zhengqi Huang, Pengpeng Ding, Chengzhi Jin, Chonglei Zhang, Lianzhong Deng, Kebin Shi, Zhenrong Sun, Xiaocong Yuan, Shian Zhang. Temporal compressive super-resolution microscopy at frame rate of 1200 frames per second and spatial resolution of 100 nm. Advanced Photonics, 2023; 5 (02) DOI: 10.1117/1.AP.5.2.026003
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